Journal: BMC Biotechnology
Article Title: pMAA-Red: a new pPZP-derived vector for fast visual screening of transgenic Arabidopsis plants at the seed stage
doi: 10.1186/1472-6750-12-37
Figure Lengend Snippet: pMAA-Red seed selection. Fluorescent seeds from transgenic Arabidopsis lines transformed with pMAA-Red ( A ) Selection of transformed seed (T 0 ) under microscope, ( B ) Silique in T 1 generation shows some non-florescent seeds (arrows) representing heterozygous line, ( C ) Silique in T 2 generation shows all florescent seeds representing homozygous line, ( D ) The selected homozygous line in T 2 generation.
Article Snippet: Seeds of transformed plants were harvested and photographed under an inverse microscope equipped with a DsRed fluorescence filter (Axiovert 200 M; Zeiss, Hallerbergmoos, Germany) and an integrated camera (AxioCam MRc5; Zeiss).
Techniques: Selection, Transgenic Assay, Transformation Assay, Microscopy